Conformational Analysis of a DNA Triplet in Aqueous Solution
- 1 December 1976
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 71 (2), 557-562
- https://doi.org/10.1111/j.1432-1033.1976.tb11145.x
Abstract
The proton signals of a 50 mM solution of thymidylyl-(3''-5'')-thymidylyl-(3''-5'')-2''-deoxyadenosine, d(T-T-A), in 2H2O at 65.degree. C and p2H = 7.4, were detected by NMR at 360 NHz. Complete assignment of the 21 ribose proton signals was achieved by extensive decoupling experiments and computer simulations. The coupling constant data show that the ribose rings prefer to adopt the S (2''-endo) conformation. The presence of a substantial amount of a right handed helical structure of the d(-T-A) fragment is indicated from chemical shift considerations. The molecule displays a high degree of conformational purity along the sugar-phosphate backbone.This publication has 17 references indexed in Scilit:
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