Clones of Individual Repetitive Sequences from Sea Urchin DNA Constructed with Synthetic Eco RI Sites
- 8 April 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 196 (4286), 197-200
- https://doi.org/10.1126/science.847467
Abstract
Interspersed repetitive sequences were isolated from sea urchin DNA by renaturing to low Cot followed by treatment with nuclease SI. Synthetic Eco RI sites were ligated onto the repetitive sequence elements, which were then inserted at the Eco RI site of plasmid RSF2124 and cloned. The repetitive sequences can be excised from the plasmid with Eco RI for further study.This publication has 11 references indexed in Scilit:
- Exploration of long and short repetitive sequence relationships in the sea urchin genomeNucleic Acids Research, 1977
- Chemical Synthesis of Restriction Enzyme Recognition Sites Useful for CloningScience, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- A general method for inserting specific DNA sequences into cloning vehiclesGene, 1976
- Synthetic lacoperator DNA is functional in vivoNature, 1976
- The generation of a colE1-Apr cloning vehicle which allows detection of inserted DNAMolecular Genetics and Genomics, 1975
- Interspersion of repetitive and non-repetitive DNA sequences in the sea urchin genomeCell, 1974
- Studies on Polynucleotides, C A Novel Joining Reaction Catalyzed by the T4-Polynucleotide LigaseProceedings of the National Academy of Sciences, 1970
- A nuclease specific for heat-denatured DNA isolated from a product of Aspergillus oryzaeBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
- ColicinsAnnual Review of Microbiology, 1957