Identification and Characterization of Sulfhydryl-Containing Proteolytic Fragments Involved in the Ca2+-Induced Conformational Change of Beef Brain S-100
- 1 September 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 39 (3), 601-612
- https://doi.org/10.1111/j.1471-4159.1982.tb07936.x
Abstract
The Ca2+-dependent conformational alteration of the brain-specific S-100 protein was studied by reacting the protein with N-ethyl[2,3-14C]maleimide in the absence and presence of Ca2+ and under denaturing conditions. Peptic hydrolysates of the 14C-labeled protein were analyzed and fractionated by high-performance liquid chromatography. Labeled peptide fractions were characterized by high-voltage electrophoresis and TLC. A clear distinction could be made between 2 classes of sulfhydryl-containing fragments: neutral and hydrophobic, and acidic. Ca2+ markedly favored 14C incorporation into the former components, whereas the latter were readily available only under denaturing conditions.Keywords
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