MORPHOLOGICAL EXAMINATIONS OF MURINE GRANULOCYTE MACROPHAGE COLONIES GROWN IN SERUM-FREE CULTURES

Abstract

Murine bone marrow granulocyte/macrophage progenitor cells proliferated to form colonies consisting of their progeny in serum-free agar cultures in the presence of colony-stimulating activity. The cloning efficiency for the serum-free cultures was 80% of that for control cultures containing 20% fetal calf serum. Examinations of the cellular compositions of individual colonies revealed that the number of pure macrophage colonies was indistinguishable in the 2 culture systems while that of pure granulocyte and granulocyte/macrophage mixed colonies was smaller in the serum-free cultures.