Characterization of the genes coding for theEcoRV restriction and modification system ofEscherichia coli
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (8), 3659-3676
- https://doi.org/10.1093/nar/12.8.3659
Abstract
A plasmid encoding the recently described Eco RV restriction and modification system has been isolated and characterized. This plasmid, pLB1 , is 6.2 kb long and carries only the Eco RV genes. A subclone of 3 kb has been inserted in pBR322. The relative positions of the endonuclease and the methylase genes were determined by the construction of a set of overlapping deletions generated by Bal31 resection. The DNA sequence of a 2.2 kb fragment containing the two genes was determined. The two genes are transcribed divergently from a 310 bp region and the assignment of the coding region has been confirmed by direct aminoacid sequence analysis. Possible mechanisms of regulation of the endonuclease gene expression at the translational level are proposed and discussed.Keywords
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