Distribution among tissues and intracellular localization of cofilin, a 21kDa actin-binding protein.
- 1 January 1987
- journal article
- research article
- Published by Japan Society for Cell Biology in Cell Structure and Function
- Vol. 12 (5), 443-452
- https://doi.org/10.1247/csf.12.443
Abstract
Cofilin, a 21kDa actin-binding protein, binds to F-actin in a 1:1 molar ratio of cofilin to actin molecule (Nishida, E., S. Maekawa, and H. Sakai, Biochemistry, 23, 5307-5313, 1984) and is capable of controlling actin polymerization and dephosphorylation in vitro in a pH-sensitive manner (Yonezawa, N., E. Nishida, and H. Sakai, J. Biol. Chem., 260, 14410-14412, 1985). In this study, immunoblot analysis using monospecific antibodies against cofilin showed that cofilin is ubiquitously distributed in a variety of bovine and rat organs and tissues. Cofilin is also present in various cultured cell lines. Indirect immunofluorescence staining of mouse fibroblastic cells and human epidermoid carcinoma cells indicated that cofilin is distributed nearly uniformly in the cytoplasm and is concentrated in ruffling membranes where F-actin is also concentrated as revealed by staining with the anti-cofilin antibody, although stress fiber staining was sometimes observed near the cell periphery in mouse 3T3 cells. These results suggest that the bulk of cofilin may not be associated with F-actin bundles in vivo.This publication has 14 references indexed in Scilit:
- Opposite effects of cofilin and profilin from porcine brain on rate of exchange of actin-bound adenosine 5'-triphosphateBiochemistry, 1985
- Purification of Cofilin, a 21,000 Molecular Weight Actin-Binding Protein, from Porcine Kidney and Identification of the Cofilin-Binding Site in the Actin Sequence1The Journal of Biochemistry, 1985
- Phorbol ester and diacylglycerol mimic growth factors in raising cytoplasmic pHNature, 1984
- Cofilin, a protein in porcine brain that binds to actin filaments and inhibits their interactions with myosin and tropomyosinBiochemistry, 1984
- Epithelial cytoskeletal framework and nuclear matrix-intermediate filament scaffold: three-dimensional organization and protein composition.The Journal of cell biology, 1984
- Insulin-induced formation of ruffling membranes of KB cells and its correlation with enhancement of amino acid transport.The Journal of cell biology, 1984
- Isolation of Low Molecular Weight Actin-Binding Proteins from Porcine Brain1The Journal of Biochemistry, 1984
- Na+/H+ antiport in Swiss 3T3 cells: mitogenic stimulation leads to cytoplasmic alkalinization.Proceedings of the National Academy of Sciences, 1982
- Fluorescent phallotoxin, a tool for the visualization of cellular actin.Proceedings of the National Academy of Sciences, 1979
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979