Partial denaturation mapping of cloned histone DNA from the sea urchin Psammechinus miliaris
- 1 November 1976
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 264 (5581), 31-34
- https://doi.org/10.1038/264031a0
Abstract
A cloned 5.6-kb DNA repeat unit 0f Psammechinus miliaris containing all five histone coding sequences of known order and polarity has been shown to fall clearly into a low melting and a high melting half by thermal denaturation. The topologies of the DNA sequences thus defined were determined by partial denaturation mapping in the electron microscope to gain further insight into the distribution of spacers and genes within histone DNA.Keywords
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