DNA synthesis during the early stage of germination in the barley embryo meristems and its inhibition by N-methyl-N-nitrosourea
- 1 May 1974
- journal article
- Published by Institute of Experimental Botany in Biologia plantarum
- Vol. 16 (3), 168-173
- https://doi.org/10.1007/bf02920911
Abstract
3 peaks of DNA synthesis were observed in the barley embryo of seeds, germinating for 49 h in running tap water at 25°C. The first peak, found after 22h, was formed by S-cells in the roots and in the 1st leaf meristem. The second peak (after 34–37h) and third peak (after 46–49 h) represents the S-cells in the roots, apex and 1st, 2nd and 3rd leaf meristems. Application of N-methyl-N-nitrosourea for 3 h at the onset of germination inhibited the rate of DNA synthesis and postponed the peaks of DNA synthesis in individual meristems of the embryo. Při klíčení semen ječmene po dobu 49 h v tekoucí vodě teploty 25°C, byly zjištěny 3 vrcholy syntézy DNA v embryu. První vrchol, zjištěný po 22 h, tvořily S-buňky v kořínku a v prvním listovém meristému. Na druhém vrcholu, zjištěném po 34–37 h a na třetím vrcholu, zjištěném po 46–49 h, se podílely S-buňky v kořenovém a vrcholovém meristému a v prvním, druhém a třetím listovém meristému. Po 3 h působení N-methyl-N-nitrosomoěoviny na začátku klíčení, dochází kinhibici syntézy DNA a ke zpoždění v začátku této syntézy v jednotlivých meristémech embrya.Keywords
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