New Principles of Ion-Exchange Techniques Suitable to Sample Preparation and Group Separation of Natural Products Prior to Liquid Chromatography
- 1 April 1984
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 7 (4), 691-707
- https://doi.org/10.1080/01483918408073996
Abstract
A Gentle method of group separation of low molecular weight hydrophilic natural products is reported. The method is based on separation of the compounds according to their net charge at different pH values using different types of ion-exchange columns connected in series. Precolumns retaining interfering compounds are used in some cases. Elution of the compounds retained on the columns is performed by use of volatile eluents. The elution principle for two of the ion-exchangers in question is removal of the charges on the column materials while for the third column the positive net charge on the compounds retained is removed. Thereby, the total amount of ions retained on the different columns is released and eluted into small volumes, which after evaporation leaves the ions as well defined salts. The method is experimentally simple and efficient to separation of natural products into groups suitable to direct use in sensitive methods of analysis as e.g. high-performance liquid chromatography and gas chromatography. Combinations of these column chromatographic methods have been adpated for micro or semimicro determinations of naturally occurring compounds, e.g., aromatic choline esters, amines, amino acids and esters of phenolic carboxylic acids. The methods seem to be general practicable for group separation of low molecular weight hydrophilic compounds.Keywords
This publication has 14 references indexed in Scilit:
- Separation of aromatic choline esters by high-performance liquid chromatographyJournal of Chromatography A, 1983
- Development of 1-O-sinapoyl-?-D-glucose: L-malate sinapoyltransferase activity in cotyledons of red radish (Raphanus sativus L. var. sativus)Planta, 1982
- Separation and quantification of basic hydroxycinnamic amides and hydroxycinnamic acids by reversed-phase high-performance liquid chromatographyJournal of Chromatography A, 1982
- Determination of Vicine and Convicine in Fababeans by High Pressure Liquid ChromatographyCanadian Institute of Food Science and Technology Journal, 1982
- 4-Hydroxybenzoylcholine: A natural product present in Sinapis albaPhytochemistry, 1982
- Recent advances in the analysis of glucosinolatesJournal of Oil & Fat Industries, 1981
- Rapid reversed-phase high-performance liquid chromatography method for the quantitation of vicine, convicine and related compoundsJournal of Chromatography A, 1981
- New Methods of Quantitative Analysis of GlucosinolatesPublished by Springer Nature ,1981
- Separation of glucosinolates by high-performance liquid chromatographyJournal of Chromatography A, 1980
- Isolation of glucosinolates and the identification of o-(α-l-rhamnopyranosyloxy)benzylglucosinolate from Reseda odorataPhytochemistry, 1979