Fluorometric liquid chromatographic determination of aliphatic aldehydes arising from lipid peroxides.
- 1 January 1986
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 34 (4), 1694-1700
- https://doi.org/10.1248/cpb.34.1694
Abstract
A fluorometric method for the determination of aliphatic aldehydes using 1,3-cyclohexanedione reagent and high-performance liquid chromatography (HPLC) was developed. Straight-chain aldehydes of C3-C10 were reacted with 1,3-cyclohexanedione at 60.degree. C for 1 h and the fluorescent decahydroacridine derivatives2.3) formed were determined by HPLC on an ODS column. The calibration curves for the aldehydes were linear in the range of 0-10 ng and the relative standard deviation at the 5 ng level was about 2.3%. This method was sensitive enough to measure the amount of aliphatic aldehydes, including 4-hydroxynonenal, generated by microsomal peroxidation in rat liver.This publication has 5 references indexed in Scilit:
- Trace analysis of aldehydes by reversed-phase high-performance liquid chromatography and precolumn fluorigenic labeling with 5,5-dimethyl-1,3-cyclohexanedioneJournal of Chromatography A, 1983
- Detection of carbonyl functions in phospholipids of liver microsomes in CCl4- and BrCCl3-poisoned ratsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982
- Identification of 4-hydroxynonenal as a cytotoxic product originating from the peroxidation of liver microsomal lipidsBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1980
- HUMAN ARTERIAL AND VENOUS TISSUES GENERATE PROSTACYCLIN (PROSTAGLANDIN X), A POTENT INHIBITOR OF PLATELET AGGREGATIONThe Lancet, 1977
- Autoxidation of polyunsaturated esters in water: chemical structure and biological activity of the productsJournal of Lipid Research, 1967