Sensitive, Rapid, and Specific Bioassay for the Determination of Antilipogenic Compounds
Open Access
- 1 September 1977
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 12 (3), 308-313
- https://doi.org/10.1128/aac.12.3.308
Abstract
A sensitive and rapid bioassay for the determination of the antilipogenic compounds cerulenin and CM-55 is described. The bioassay is based on the inhibitory effect of cerulenin and CM-55 on the in vivo luminescence of an aldehyde-requiring mutant of the marine bacterium Beneckea harveyi . A total quantity as low as 0.1 μg of cerulenin can be determined within 15 min with an error of ±2%. The bioassay, as presented, is specific for compounds that are known to inhibit fatty acid biosynthesis and, as such, it might be used as a general screening method for the detection of antilipogenic compounds.Keywords
This publication has 9 references indexed in Scilit:
- The antibiotic cerulenin, a novel tool for biochemistry as an inhibitor of fatty acid synthesis.1976
- Mechanism of Action of CM-55, a Synthetic Analogue of the Antilipogenic Antibiotic CeruleninAntimicrobial Agents and Chemotherapy, 1974
- Target of inhibition by the anti-lipogenic antibiotic cerulenin of sterol synthesis in yeastBiochemical and Biophysical Research Communications, 1974
- REVISED STRUCTURE OF CERULENINThe Journal of Antibiotics, 1974
- Inhibition of fatty acid synthesis by the antibiotic ceruleninBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1973
- Inhibition of fatty acid synthetases by the antibiotic ceruleninBiochemical and Biophysical Research Communications, 1972
- The Action Mechanism of CeruleninThe Journal of Biochemistry, 1972
- The Inhibition of Bacterial Luciferase by Mixed Function Oxidase InhibitorsJournal of Biological Chemistry, 1972
- Determination of subpicomole levels of NADH and FMN using bacterial luciferase and the liquid scintillation spectrometerAnalytical Biochemistry, 1971