Untargeted mutagenesis induced by UV in the lacI gene of Escherichia coli
- 1 September 1985
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 201 (1), 30-34
- https://doi.org/10.1007/bf00397982
Abstract
Using a nonselective method, we have estimated the proportion of untargeted mutations in the lacI gene of E. coli by transferring either irradiated or unirradiated F′ pro lac plasmids from an excision deficient donor to an excision deficient pro lac deleted recipient that had been irradiated and allowed to induce recA dependent functions for 30 min. We find that about 10 percent of the mutations induced by either 3.5 Jm-2 or 7 Jm-2 UV are untargeted.This publication has 31 references indexed in Scilit:
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