A Guide to Thin-Layer Chromatographic Systems for the Separation of Aflatoxin B1, B2, G1and G2

Abstract
Separation of aflatoxins B1, B2, G1 and G2 were compared on 6 commercial silica gel plates in 12 solvent systems. Two solvent systems, chloroform:acetone:ammonium hydroxide (90:10:0.25) and chloroform: acetone:hexane (85:15:20), desolved the 4 aflatoxins on all tested plates. The solvent modifier played an important role in the resolution of these compounds. The effect of the hardness of the plate was also discussed.