Binding of Disaccharides by Peanut Agglutinin as Studied by Ultraviolet Difference Spectroscopy
Open Access
- 1 April 1982
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 123 (2), 305-310
- https://doi.org/10.1111/j.1432-1033.1982.tb19768.x
Abstract
The binding of the disaccharides methyl β-d-lactoside and 2-acetamido-2-deoxy-3-O-(β-d-galactopyranosyl)- β-dD-galactopyranose [β-d-Gal-(l→3)-d-GalNAc] to peanut agglutinin was studied by ultraviolet difference spectroscopy. The magnitude of the difference spectra varied with the concentration of the carbohydrates; association constants and thermodynamic parameters were determined from titration experiments at different temperatures. The enthalpy and entropy changes for binding of methyl β-d-lactoside were found to be ΔH°= -65 ± 4 kJ mol−1, ΔSdeg; =−156 ± 14 J mol−1 K−1. For β-dD-Gal- (1→3)-d-Gal NAc the observed thermodynamic parameters were ΔH°=−78 ± kJ mol−1, ΔS°=−177 ± 16 J mol−1 K−1. For both disaccharides, the enthalpy change upon binding to the lectin is much larger than that found for the binding of methyl α- and β-d-galactopyranosides, strongly suggesting the existence of an extended binding site on peanut agglutinin. The observed parameters are compared with those found for the binding of monosaccharides and oligosaccharides to other lectins and to lysozymc. Molecular models of the minimum energy conformers of β-GaI-(1→3)-d-GalNAc and methyl β-d-Iactoside are used to interpret the interaction of these, and structurally related ligands, with the peanut agglutinin binding site.This publication has 31 references indexed in Scilit:
- Carbon-13 nuclear magnetic resonance studies on lectin-carbohydrate interactions: binding of specifically carbon-13-labeled methyl .beta.-D-lactoside to peanut agglutininBiochemistry, 1982
- Structure de la N-acétyllactosamine (acétamido-2 désoxy-2 O-β-D-galactopyrannosyl-4a-D-glucopyrannose)Acta Crystallographica Section B: Structural Science, Crystal Engineering and Materials, 1981
- Binding Kinetics of 4‐Methylumbelliferyl α‐Mannobioside to Concanavalin A by Fluorescence Stopped‐Flow MeasurementsEuropean Journal of Biochemistry, 1980
- Interaction entre l'hémagglutinine de ricin et ses ligands, galactose et lactose. Etude par microcalorimétrie et dialyse à l'équilibreBiochimie, 1979
- Chondroitin 4-sulfate: The structure of a sulfated glycosaminoglycanJournal of Molecular Biology, 1978
- Binding of 4-Methylumbelliferyl alpha-Manno-oligosaccharides to Concanavalin A: Equilibrium StudyEuropean Journal of Biochemistry, 1978
- A charge-density study of crystalline berylliumActa Crystallographica Section A, 1977
- Common precursors of human blood group MN specificitiesBiochemical and Biophysical Research Communications, 1974
- A Reinvestigation of the Preparation of β-Methyl Lactoside1,2Journal of the American Chemical Society, 1952
- RELATIONS BETWEEN ROTATORY POWER AND STRUCTURE IN THE SUGAR GROUP. X.2 THE CHLORO-, BROMO- AND IODO-ACETYL DERIVATIVES OF LACTOSEJournal of the American Chemical Society, 1925