Antitermination is required for readthrough transcription of the maize rbcL gene by a bacteriophage promoter in Escherichia coli

Abstract

Sequences upstream of the 5'' end of the rbcL gene in maize chloroplast fragment Bam 9, have a polar effect on the expression of rbcL from an external upstream bacteriophage PL promoter in Escherichia coli. This polarity can be suppressed by the bacteriophage transcription antitermination protien N or Q. The requirement for transcriotion antitermination is abolished if DNA upstream of rbcL is removed by a deletion. We have also investigated the ability of RNA polymerase initiating transcription at PL in the presence of N to transcribe through the normal rbcL transcription terminator and into sequences beyond. RNA polymerase initiating at PL can traverse rbcL and its 5'' and 3''-flanking regions in the presence of N.