Amino‐Acid Sequence of the L‐1 Light Chain of Chicken Cardiac‐Muscle Myosin
- 1 June 1980
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 107 (2), 565-575
- https://doi.org/10.1111/j.1432-1033.1980.tb06064.x
Abstract
The light chain fraction was separated from myosin extracted from chicken cardiac muscle. Two light chain components, L-1 and L-2 in the fraction were isolated by chromatography on a column of DEAE-cellulose (DE-52) in the presence of 4 M urea. After performic acid oxidation, the L-1 chain was digested with trypsin and the resulting peptides were isolated. The amino acid sequences of the peptides were established. The ordering of these tryptic peptides in the L-1 chain was deduced from the amino acid compositions and the partial sequences of peptic peptides from S-carboxymethylated L-1 chain. Comparing the whole sequence of the L-1 chain thus established with that of alkali light chain of rabbit skeletal muscle myosin, 67 amino acid substitutions and 2 insertions were recognized.This publication has 17 references indexed in Scilit:
- The Amino Acid Sequence of the L-2 Light Chain of Chicken Skeletal Muscle MyosinHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1980
- The L‐2 light chain of chicken skeletal muscle myosinFEBS Letters, 1977
- The Amino-Acid Sequence of the Alkali Light Chains of Rabbit Skeletal-Muscle MyosinEuropean Journal of Biochemistry, 1974
- THE PRIMARY STRUCTURES OF α AND β CHAINS OF ADULT HEMOGLOBIN OF THE JAPANESE MONKEY (Macata fuscata fuscata)International Journal of Peptide and Protein Research, 1973
- Light Chains from Fast and Slow Muscle MyosinsNature, 1971
- AMINO ACID SEQUENCES OF ALL THE TRYPTIC PEPTIDES FROM THE α POLYPEPTIDE CHAIN OF ADULT HEMOGLOBIN OF THE RHESUS MONKEY (Macaca mulatto)International Journal of Protein Research, 1970
- Strategy and tactics in protein chemistryBiochemical Journal, 1970
- High recovery of tryptophan from acid hydrolysates of proteinsBiochemical and Biophysical Research Communications, 1969
- Electrophoretic Mobilities of Peptides on Paper and their Use in the Determination of Amide GroupsNature, 1966
- Colour Reactions on Paper Chromatograms by a Dipping TechniqueNature, 1953