A Turbidimetric Assay for Tyrosinase Activity in Frozen Sections of Mouse Skin.

Abstract

A new sensitive and objective method for the turbidimetric assay of tyrosinase activity in frozen skin sections is described. The procedure involves the dispersion of both melanin and skin and hair keratin, from previously incubated sections, in hot molar Na2S and KOH. Turbidimetric readings are then obtained on the absolute scale of the Klett-Summerson photoelectric colorimeter. This method is immediately useful for determining order of effect, and, if sufficiently improved, might also be useful for comparing amounts of naturally occurring pigment in different skin and hair samples.