The Enzyme Linked Immunosorbent Assay (ELISA) was compared with the indirect immunofluorescence antibody test (IFAT) and with the counter-immunoelectrophoresis (CIE) in the serology of amebiasis. In cases of liver abscesses, the ELISA was as sensitive as CIE, but slightly more sensitive than IFAT. In cases where the patient asymptomatically passed cysts of E. histolytica, none of the three tests was positive. As a routine laboratory screening test, the ELISA proved to be the most convenient of the three.