Juvenile hormone-binding protein from the cytosol of Drosophila Kc cells
- 1 August 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (8), 4657-4661
- https://doi.org/10.1073/pnas.77.8.4657
Abstract
Insect cells of an established line, D. melanogaster Kc cells, take up and metabolize juvenile hormone (JH). The cytoplasm of these cells contains a protein that binds JH with specificity, saturability and high affinity (Kd = 1.56 .times. 10-8 M). The kinetics for the specific binding and dissociation of JH I were independently measured, and the rate constants were Ka = 1.3 .times. 106 M-1 min-1, Kd = 1.3 .times. 10-2 min-1, respectively. All 3 juvenile hormones bind to the protein with comparable affinities; the corresponding acid or diol metabolites of JH I are not bound. About 2500 hormone-binding protein molecules are present/cell. The protein has a MW of 80,000 as estimated by gel permeation chromatography and by sucrose gradient sedimentation. The properties of this protein suggest that it functions as a cytoplasmic receptor for JH.This publication has 17 references indexed in Scilit:
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