Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana

Abstract

The plant Arabidopsis thaliana (Arabidopsis) has become an important model species for the study of many aspects of plant biology¹. The relatively small size of the nuclear genome and the availability of extensive physical maps of the five chromosomes^2,3,4 provide a feasible basis for initiating sequencing of the five chromosomes. The YAC (yeast artificial chromosome)-based physical map of chromosome 4 was used to construct a sequence-ready map of cosmid and BAC (bacterial artificial chromosome) clones covering a 1.9-megabase (Mb) contiguous region⁵, and the sequence of this region is reported here. Analysis of the sequence revealed an average gene density of one gene every 4.8 kilobases (kb), and 54% of the predicted genes had significant similarity to known genes. Other interesting features were found, such as the sequence of a disease-resistance gene locus, the distribution of retroelements, the frequent occurrence of clustered gene families, and the sequence of several classes of genes not previously encountered in plants.