Rapid Isolation of Clostridiums by Selective Inhibition of Aerobic Flora. I I . A Systematic Method as Applied to Surveys of Clostridiums in Korea

Abstract

Two inhibitory media, sorbic acid polymyxin B sulfate-thioglycollate broth and chloral hydrate-sodium azide blood agar plate in combination, together with 2 noninhibitory media, thioglycollate broth and blood agar plates, were evaluated. This method was used as a routine procedure for the isolation of the bacterial flora and, in particular, for the members of the genus Clostridium from clinical material received from Korea. The results of a controlled experiment, using known type strains, demonstrated that all spp. of Clostridium. routinely encounterd, survived multiple transfers on all media used. The time necessary for isolation and separation of Clostridial species was greatly reduced as compared with other methods. A procedure for the isolation of Clostridiums from grossly contaminated materials is given.