Studies on Microsomal Glucose-6-phosphate Dehydrogenase of Rat Livers*

Abstract

The microsomal and soluble glucose-6-phosphate dehydrogenase [D-glucose-6-phosphare: NADP oxidoreductase, EC 1.1.1.49] (G6PD) in rat liver were identified by electrophoresis and an immunological procedure. No cross immunological reaction was observed between the two. The soluble G6PD demonstrated a considerable change in response to various physiological conditions as reported, while the microsomal enzyme showed no change under any of the conditions examined. The microsomal G6PD increased during fetal development and was greatly enhanced at birth and then gradually decreased to the adult level. The microsomal enzyme in regenerating liver decreased at the 2nd day after partial hepatectomy and increased at the 5th day and returned to the normal level at the 14th day. The microsomal enzyme was not significantly found in ascites hepatoma.