Evidence for apical chloride channels in rabbit mandibular salivary glands
- 1 June 1988
- journal article
- research article
- Published by Springer Nature in Pflügers Archiv - European Journal of Physiology
- Vol. 411 (6), 670-675
- https://doi.org/10.1007/bf00580864
Abstract
Double-barrelled, chloride-selective microelectrodes were used to study mandibular gland acinar cells at rest and during cholinergic stimulation. At rest, intracellular chloride activity was five times the expected equilibrium activity. During sustained stimulation with acetylcholine, chloride activity fell to three times the expected equilibrium activity. Thus, the gradient for chloride exit was reduced in the stimulated cell. These results lead to the conclusion that stimulation increases the permeability of the acinar cell to chloride. Experiments in which extracellular chloride was removed provided evidence that the permeability increase was due to opening of chloride channels located principally in the apical membrane of the acinar cell.This publication has 23 references indexed in Scilit:
- Acetylcholine stimulates a Ca2+-dependent Cl? conductance in mouse lacrimal acinar cellsPflügers Archiv - European Journal of Physiology, 1985
- Effects of 4,4′-diisothiocyano-2,2′-stilbene disulphonic acid and amiloride on salivary secretion by isolated, perfused rat submandibular glandsArchives of Oral Biology, 1985
- Mechanism of NaCl secretion in rectal gland tubules of spiny dogfish (Squalus acanthias)Pflügers Archiv - European Journal of Physiology, 1984
- The dependence of fluid secretion by mandibular salivary gland and pancreas on extracellular calciumCell Calcium, 1983
- Effect of amiloride on electrolyte transport parameters of the main duct of the rabbit mandibular salivary glandPflügers Archiv - European Journal of Physiology, 1983
- Some properties of KCl-filled microelectrodes: Correlation of potassium “leakage” with tip resistanceThe Journal of Membrane Biology, 1981
- Electrolyte and protein secretion by the perfused rabbit mandibular gland stimulated with acetylcholine or catecholaminesThe Journal of Physiology, 1980
- Calcium ion activity in physiological salt solutions: Influence of anions substituted for chlorideComparative Biochemistry and Physiology Part A: Physiology, 1975
- Ionic hydration and single ion activities in unassociated chlorides at high ionic strengthsAnalytical Chemistry, 1970
- The effect of sodium ions on the electrical activity of the giant axon of the squidThe Journal of Physiology, 1949