New Techniques for Qligosaccharide Sequencing

Abstract
A new procedure is described for oligosaccharide sequencing that combines HPLC [high performance liquid chromatography] purification, the sensitive technique of fluorescence detection and partial polymer degradation by ionic hydrogenation with component determination by direct chemical ionization mass spectrometry (DCI-MS) and GC-MS [gas chromatography-mass spectrometry]. The methodology is demonstrated for a series of model compounds and provides linkage information as well as sugar sequence.