Über die Umwandlung von Fett in Kohlehydrat. I. Mitteilung. Über die Phosphatide als Vorstufen der Fettoxydation. (Nach Versuchen an der isolierten Leber.)

Abstract

Isolated dog-liver was perfused with defibrinated ox-blood plus Ringer''s solution, to which in some instances phosphatides were added. Observations were made on changes in the distribution of the phosphatides in the blood and liver, on the effect of the added phosphatides on O consumption and CO2 production, as well as on the formation of ketone bodies and sugar. Added phosphatides were quickly taken up by the liver; phosphatides already present in the blood-corpuscles were not affected. Phosphatides present in fatty-livers split off considerable amounts of phosphoric acid; this was not the case for livers high in glycogen but low in fat. Addition of phosphatides to high-glycogen livers caused an increase in O consumption and acetic acid formation, while the R.-Q. was Towered markedly. With the exception of the effect on ketone-body formation, livers high in fat from phlorhizin feeding showed similar effects. The results of the experiment were thought to indicate that a breakdown of higher fatty acids accompanies the intermediary formation ol phos-phatides. By artificial perfusion of phlorhizin livers, sugar may be formed from the fatty acid portion of the phosphatide.