Cytochemical Localization of K+-stimulated Adenosine Triphosphatase Activity in Xylem Parenchyma Cells of Barley Roots
Open Access
- 30 November 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 60 (6), 923-927
- https://doi.org/10.1104/pp.60.6.923
Abstract
ATPase activity in xylem parenchyma cells of barley (Hordeum vulgare L.) roots was demonstrated cytochemically with a lead precipitation reaction. The methodical parameters of this cytochemical test were optimized for distinction between ATPase-specific and nonspecific precipitates. Optimum conditions were prefixation in 1% glutaraldehyde for 1 hour and incubation for 2 hours in a medium containing 2 mm each of ATP, Ca2+, and Pb2+ at pH 7 and 25 C. Problems of cytochemical localizations are discussed.This publication has 8 references indexed in Scilit:
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