Norepinephrine decreases EGF binding in primary rat hepatocyte cultures

Abstract

Norepinephrine (NE) produced a dose-dependent inhibition of ¹²⁵I-epidermal growth factor (EGF) binding to adult rat hepatocytes in primary culture. This effect was maximal after 1 hr of incubation with NE and could be blocked by the presence of an α₁-specific adrenergic receptor antagonist. The inhibition of binding correlates with the ability of NE to enhance hepatocyte DNA synthesis in the presence of EGF and appears to be mediated by a reduction in EGF receptor number, without a significant change in receptor affinity.