FLUORESCEIN IN HUMAN PLASMA IN VITRO
- 1 October 1982
- journal article
- research article
- Published by Wiley in Acta Ophthalmologica
- Vol. 60 (5), 701-708
- https://doi.org/10.1111/j.1755-3768.1982.tb06730.x
Abstract
A method to determine fluorescein [used in ophthalmological diagnosis] in human plasma is described. By aid of ultrafiltration a separation between fluorescein bound to plasma proteins and fluorescein free in the water is obtained. Both fractions are quantitated. Fluorescein is bound to plasma proteins. The protein binding is reversible, not sensitive to practically appearing changes in pH, temperature and gas tensions. For a normal person it appeared that at a total plasma concentration between (10-6-10-4) g/ml, .apprx. 15% was free, while at a total concentration of 10-3 g/ml, 45% was free, pointing towards a limited amount of protein binding sites.Keywords
This publication has 11 references indexed in Scilit:
- FLUORESCEIN IN HUMAN PLASMA IN VITROActa Ophthalmologica, 1982
- THE BLOOD‐RETINAL BARRIER PERMEABILITY IN DIABETIC PATIENTSActa Ophthalmologica, 1981
- Influence of plasma protein binding kinetics on hepatic clearance assessed from a “tube” model and a “well-stirred” modelJournal of Pharmacokinetics and Biopharmaceutics, 1981
- Tritiated Fluorescein Binding to Normal Human Plasma ProteinsArchives of Ophthalmology (1950), 1980
- The blood-ocular barriersSurvey of Ophthalmology, 1979
- Kinetics of the Binding of Salicylazosulfapyridine to Human Serum AlbuminActa Pharmacologica et Toxicologica, 1977
- Studies on “Nonspecific” BindingEuropean Journal of Biochemistry, 1971
- The active transport of fluorescein by the retinal vessels and the retinaThe Journal of Physiology, 1967
- A Method of Photographing Fluorescence in Circulating Blood in the Human RetinaCirculation, 1961
- Über Fluorescein in der menschlichen VorderkammerOphthalmologica, 1950