Phosphonomethyl analogues of phosphate ester glycolytic intermediates
- 1 September 1974
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 141 (3), 715-719
- https://doi.org/10.1042/bj1410715
Abstract
Analogues of dihydroxyacetone phosphate and of 3-phosphoglycerate were made in which the phosphate group, –O–PO3H2, is replaced by the phosphonomethyl group, –CH2–PO3H2. The analogue of dihydroxyacetone phosphate is a substrate for aldolase and glycerol 1-phosphate dehydrogenase (Stribling, 1974), but not for triose phosphate isomerase. The analogue of 3-phosphoglycerate oxidizes NADH under the combined action of 3-phosphoglycerate kinase and glyceraldehyde 3-phosphate dehydrogenase if ATP is added. Thus four out of the five glycolytic enzymes tested handle the phosphonomethyl compounds like the natural phosphates.Keywords
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