31P NMR of mammalian cells encapsulated in alginate gels utilizing a new phosphate‐free perfusion medium
- 1 February 1990
- journal article
- research article
- Published by Wiley in NMR in Biomedicine
- Vol. 3 (1), 23-26
- https://doi.org/10.1002/nbm.1940030104
Abstract
A simple technique permitting long term 31P magnetic resonance spectroscopy of cultured mammalian cells is described. Cells are encapsulated in calcium alginate gels and perfused with a new phosphate-free medium designed for maintenance of high cell viability. Intracellular pH and β-NTP/Pi ratios, studied in three cell types, remained stable over a 16–20 h NMR monitoring period. The techniques described may be useful for monitoring cell metabolism with a variety of cell types.Keywords
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