Molecular Recognition Based on Low-Affinity Polyvalent Interactions: Selective Binding of a Carboxylated Polymer to Fibronectin Fibrils of Live Fibroblast Cells
- 29 May 2008
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 130 (25), 7851-7853
- https://doi.org/10.1021/ja8007402
Abstract
To explore molecular recognition of biomolecules in the complex environment of the extracellular matrix, we utilized two fluorescent poly(p-phenyleneethynylene)s bearing either cationic alkylammonium or negatively charged carboyxlate side chains. While incubation of live NIH 3T3 fibroblast cells with the cationic polymer yielded perinuclear punctate staining reminiscent of endocytotic vesicles, the carboxylated polymer revealed a characteristic filamentous staining pattern. Histochemical and immunofluorescence studies demonstrated that the anionic PPE selectively binds to fibronectin fibrils of the extracellular matrix. An in vitro binding study revealed a dissociation constant of approximately 100 nM for the fibronectin−polymer complex. Both polymers showed bright two-photon excited emission as well as low toxicity, rendering them well-suited for live cell imaging studies. The studies demonstrate that selective molecular recognition of biomolecules in the complex environment of the extracellular matrix can be achieved by means of nonspecific low-affinity polyvalent interactions.Keywords
This publication has 33 references indexed in Scilit:
- Activating B Cell Signaling with Defined Multivalent LigandsACS Chemical Biology, 2007
- Use of a Folate−PPE Conjugate To Image Cancer Cells in VitroBioconjugate Chemistry, 2007
- Selective Tumor Cell Targeting Using Low-Affinity, Multivalent InteractionsACS Chemical Biology, 2007
- On the Nature of the Multivalency Effect: A Thermodynamic ModelJournal of the American Chemical Society, 2003
- Designing a polyvalent inhibitor of anthrax toxinNature Biotechnology, 2001
- Synthetic multivalent ligands in the exploration of cell-surface interactionsCurrent Opinion in Chemical Biology, 2000
- High-Affinity Pentavalent Ligands of Escherichia coli Heat-Labile Enterotoxin by Modular Structure-Based DesignJournal of the American Chemical Society, 2000
- Shiga-like toxins are neutralized by tailored multivalent carbohydrate ligandsNature, 2000
- Inhibition of Viral Adhesion and Infection by Sialic-Acid-Conjugated Dendritic PolymersBioconjugate Chemistry, 1999
- Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and InhibitorsAngewandte Chemie International Edition, 1998