Abstract
Multiple isoaccepting tRNA''s for 20 amino acids from mouse plasma cell tumor, MOPC-31C, were resolved on a reversed phase Freon chromatography column. Multiplicity of isoaccepting tRNA''s may be related to the number of synonym codons. Heterogeneity was more extensive in this tissue than that reported for Escherichia coli. The possibilities that the isoaccepting peaks might be due to aggregation, partial degradation, or differential removal of pCpCpA terminal were virtually excluded. Charged animoacyl tRNA''s were eluted differently than the uncharged correspondents.