Gas-liquid chromatographic estimation of tolbutamide and chlorpropamide in plasma
- 1 September 1972
- journal article
- Published by Oxford University Press (OUP) in Journal of Pharmacy and Pharmacology
- Vol. 24 (9), 713-716
- https://doi.org/10.1111/j.2042-7158.1972.tb09095.x
Abstract
An improved method for the gas-liquid chromatographic estimation of tolbutamide and chlorpropamide in plasma is described. The drugs are extracted into toluene, back-extracted into alkali and methylated with dimethylsulphate. The methylated derivatives are extracted into n-hexane, concentrated by evaporation and chromatographed on 3·8% UC W98 or 1% Carbowax 20M columns. The standard deviation of replicate analyses of plasma samples containing 20–100 μg/ml of either drug was about 4%. There is no interference from known metabolites of tolbutamide or chlorpropamide.Keywords
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