Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.
- 1 March 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (3), 1437-1441
- https://doi.org/10.1073/pnas.77.3.1437
Abstract
A gene library of X. laevis was constructed from embryonic DNA partially digested with restriction endonucleases HaeIII and AluI and joined to the phage .lambda. Charon 4 cloning vector with EcoRI linkers. Nucleotide sequences from 3 of the 4 related vitellogenin genes were isolated. Two of the genes (called A1 and A2) were isolated in their entirety together with long stretches of flanking sequences. These 2 closely related vitellogenin genes have lengths of about 21 and 16 kilobases [kb], but both produce a vitellogenin mRNA of 6.3 kb.This publication has 26 references indexed in Scilit:
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