Glucocorticoid Regulation of Preproenkephalin Messenger Ribonucleic Acid in the Rat Striatum*
- 1 June 1990
- journal article
- research article
- Published by The Endocrine Society in Endocrinology
- Vol. 126 (6), 3124-3130
- https://doi.org/10.1210/endo-126-6-3124
Abstract
Glucocorticoids regulate the level of preproenkephalin mRNA expression in a number of cell systems. This study investigated the expression of preproenkephalin mRNA in the brain and its regulation by glucocorticoids in vivo. Two different methods for mRNA quantitation were employed. Total RNA isolated from dissected brain tissue was analyzed in an RNAse T2 protection assay. In addition, we have used in situ hybridization to brain sections to assess the expression of preproenkephalin mRNA. The results demonstrate that in the straitum the preproenkephalin mRNA is expressed at a high level and is regulated by glucocorticoids. There is a decrease in striatal preproenkephalin mRNA after adrenalectomy (ADX), and ADX animals replaced with corticosterone express higher levels of striatal preproenkephalin mRNA than ADX animals. By in situ hybridization we have determined that the corticosterone-induced increase in striatal preproenkephalin mRNA is evident after 16 h, but not after 2 h, of corticosterone replacement of ADX animals. Elevation of circulating corticosterone levels in intact rats by 2-3 h of restraint stress, a multiple daily stress regimen over 1-21 days, or corticosterone injection did not change the level of preproenkephalin mRNA in the striatum; however, a single 16-h restraint stress resulted in a decreased level of expression. In intact rats there was a diurnal variation in the level of striatal preproenkephalin mRNA expression; ADX resulted in a decrease in the mRNA level, but did not abolish the diurnal variation in expression. Thus, while there is clearly regulation of striatal preproenkephalin mRNA by glucocorticoids, our results provide evidence for regulation by nonglucocorticoid mechanisms as well.This publication has 18 references indexed in Scilit:
- PROENKEPHALIN-A GENE-REGULATION IN THE RAT STRIATUM - INFLUENCE OF LITHIUM AND HALOPERIDOL1986
- Activation of the human beta-interferon gene requires an interferon-inducible factor.Molecular and Cellular Biology, 1986
- Permissive effect of dexamethasone on the increase of proenkephalin mRNA induced by depolarization of chromaffin cells.Proceedings of the National Academy of Sciences, 1986
- Mapping of Glucocorticoid Receptor Immunoreactive Neurons in the Rat Tel- and Diencephalon Using a Monoclonal Antibody against Rat Liver Glucocorticoid Receptor*Endocrinology, 1985
- Rat brain preproenkephalin mRNA. cDNA cloning, primary structure, and distribution in the central nervous system.Journal of Biological Chemistry, 1984
- The effect of apomorphine on the open-field behavior of rats: Alone and in pairsPharmacology Biochemistry and Behavior, 1984
- Increase of proenkephalin mRNA and enkephalin content of rat striatum after daily injection of haloperidol for 2 to 3 weeks.Proceedings of the National Academy of Sciences, 1983
- Behavioral activity of some novel aporphines in rats with 6-hydroxydopamine lesions of caudate or nucleus accumbensEuropean Journal of Pharmacology, 1983
- Adrenalectomy, dexamethasone or stress alters opioid peptides levels in rat anterior pituitary but not intermediate lobe or brainLife Sciences, 1979
- Diurnal variation in the dopamine level of rat brain areas: Effect of sodium phenobarbitalLife Sciences, 1979