Specific Targeting of a Plasmodesmal Protein Affecting Cell-to-Cell Communication

Abstract

Plasmodesmata provide the cytoplasmic conduits for cell-to-cell communication throughout plant tissues and participate in a diverse set of non–cell-autonomous functions. Despite their central role in growth and development and defence, resolving their modus operandi remains a major challenge in plant biology. Features of protein sequences and/or structure that determine protein targeting to plasmodesmata were previously unknown. We identify here a novel family of plasmodesmata-located proteins (called PDLP1) whose members have the features of type I membrane receptor-like proteins. We focus our studies on the first identified type member (namely At5g43980, or PDLP1a) and show that, following its altered expression, it is effective in modulating cell-to-cell trafficking. PDLP1a is targeted to plasmodesmata via the secretory pathway in a Brefeldin A–sensitive and COPII-dependent manner, and resides at plasmodesmata with its C-terminus in the cytoplasmic domain and its N-terminus in the apoplast. Using a deletion analysis, we show that the single transmembrane domain (TMD) of PDLP1a contains all the information necessary for intracellular targeting of this type I membrane protein to plasmodesmata, such that the TMD can be used to target heterologous proteins to this location. These studies identify a new family of plasmodesmal proteins that affect cell-to-cell communication. They exhibit a mode of intracellular trafficking and targeting novel for plant biology and provide technological opportunities for targeting different proteins to plasmodesmata to aid in plasmodesmal characterisation. In plants, cylindrical, microscopic channels called plasmodesmata provide intracellular connections between cells for communication and material transport, and are important for many aspects of plant growth and defence. We identify a novel family of plasmodesmata-located proteins (called PDLP1) with features of type I membrane receptor-like proteins. In line with the potential for this protein to regulate molecular movement from cell to cell, we show that altered expression of the protein changes the efficiency of protein diffusion from plasmodesmata. We have also analysed the manner in which PDLP1 is transported to plasmodesmata. We show that the single transmembrane domain (TMD) of the protein contains all the information necessary for targeting to plasmodesmata and that proper targeting depends upon specific interactions with other factors within the membrane. Notably, a single amino acid close to the C-terminus of the TMD is critical for determining the intracellular destination. Further, by fusing the TMD to yellow fluorescent protein, we establish that the TMD can be used to target heterologous proteins to plasmodesmata.