Expression of TWIK-1, a novel weakly inward rectifying potassium channel in rat kidney

Abstract

Several K⁺ conductances have been identified in the kidney, with specific properties and localization in distinct cell types and membrane domains. On the other hand, several K⁺ channels have been characterized at the molecular level. By immunolocalization, we show that a new inward rectifying K⁺channel, TWIK-1, is specifically expressed in distinct tubular segments and cell types of the rat kidney. In the proximal tubule, TWIK-1 prevails in the initial portions (convoluted part), where it is restricted to the apical (brush-border) membrane. In the collecting duct, immunofluorescence was intracellular or confined to the apical membrane and restricted to intercalated cells, i.e., in cells lacking aquaporin-2, as shown by double immunofluorescence. TWIK was also expressed in medullary and cortical parts of the thick limb of the loop of Henle, identified with an anti-Tamm-Horsfall protein antibody (double immunofluorescence). The intensity of TWIK-1 immunolabeling was unchanged in rats fed a low-Na⁺ or a low-K⁺ diet. Because TWIK-1 shares common properties with the low-conductance apical K⁺ channel of the collecting duct, we propose that it could play a role in K⁺ secretion, complementary to ROMK, another recently characterized K⁺ channel located in principal cells of the cortical collecting duct and in the loop of Henle.