The initiation of chromosome replication in a dnaAts46 and a dnaA + strain at various temperatures
- 1 July 1981
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 182 (2), 364-366
- https://doi.org/10.1007/bf00269686
Abstract
The regulation of chromosome replication initiation was studied at various temperatures with an E. coli dnaA46 strain and its dnaA + parent. We find that, in both strains, the “initiation mass” varies depending upon growth temperature while the replication time remains constant relative to the cell doubling time. In the permissive temperature range, the initiation mass of the dnaA46 mutant strain is larger by a constant factor than that for a dnaA + strain. We conclude that, even at temperatures permissive for growth of the dnaA46 strain, the activity of the dnaA46 product is lower than that of the wild-type protein. The dnaA gene product, therefore, plays an important role in regulating initiation.This publication has 22 references indexed in Scilit:
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