In-vivo effects of anti-inflammatory and other drugs on granulocyte emigration in the rabbit skin collection chamber

Abstract

A method for measuring localized leukocyte mobilization under in-vivo conditions with a plastic skin collection-chamber adapted to the rabbit ear was used for assessing the effects of anti-inflammatory and other agents on granulocyte emigration. Studies on the effect of oral drug administration to rabbits indicated that most anti-inflammatory drugs, 2 cystostatic agents (cyclophosphamide and colchicine), but none of the other compounds exhibiting antihistaminic, .beta.-adrenolytic or neuroleptic properties, significantly inhibited granulocyte mobilization. The results after topical application of some of these agents into the chamber correlated well with those obtained after oral treatment. This technique may thus prove useful in selecting new compounds inhibiting granulocyte mobilization in acute inflammatory reactions.