Formation of cartilage tissue in vitro
- 1 March 1991
- journal article
- review article
- Published by Wiley in Journal of Cellular Biochemistry
- Vol. 45 (3), 258-260
- https://doi.org/10.1002/jcb.240450306
Abstract
Articular cartilage is notoriously defective in its capacity for self‐repair, making joints particularly sensitive to degenerative processes. However, methods are now available for the preparation of large numbers of differentiated chondrocytes from a small biopsy sample from any patient. The cells are amplified by proliferation as fibroblast‐like cells that will re‐express the cartilage phenotype when placed in suspension or gel culture. The chondrocytes can be collected from gel cultures after agarase treatment and reconstituted into cartilage tissue in pellet cultures. In addition, these chondrocytes can be suspended in an appropriate delivery vehicle and implanted into defect sites with a high reparative success rate in an animal model. Appropriate procedures can now be tested in appropriate patient populations.Keywords
This publication has 21 references indexed in Scilit:
- Transforming growth factor-beta and the initiation of chondrogenesis and osteogenesis in the rat femur.The Journal of cell biology, 1990
- Novel Regulators of Bone Formation: Molecular Clones and ActivitiesScience, 1988
- Prolonged expression of differentiated phenotype by chondrocytes cultured at low density on a composite substrate of collagen and agarose that restricts cell spreadingDifferentiation, 1988
- Differences between sub-populations of cultured bovine articular chondrocytes. I. Morphology and cartilage matrix productionConnective Tissue Research, 1988
- Evidence for sequential appearance of cartilage matrix proteins in developing mouse limbs and in cultures of mouse mesenchymal cellsDifferentiation, 1987
- The Repair of Major Osteochondral Defects in Joint Surfaces by Neochondrogenesis with Autogenous Osteoperiosteal Grafts Stimulated by Continuous Passive MotionClinical Orthopaedics and Related Research, 1986
- In-Vitro Culture of Human Chondrocytes From Adult SubjectsmConnective Tissue Research, 1986
- Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gelsCell, 1982
- THE GROWTH OF CARTILAGE CELLS IN SOFT AGAR AND LIQUID SUSPENSIONThe Journal of cell biology, 1970
- Isolation and culture of chondrocytes from human adult articular cartilageArthritis & Rheumatism, 1967