Acyltransferase Activity to 1-O-Alkyl-Glycero-3-phosphorylcholine in Sarcoplasmic Reticulum

Abstract

1. The activity acylating l-O-alkyl-glycero-3-phosphorylcholine was found for the first time in rabbit sarcoplasmic reticulum, which also exhibits acyl transfer activities to other phosphoglycerides. The product of the enzymatic reaction was proved to be l-O-alkyl-2-acyl-glycero-3-phosphorylcholine. 2. The acyl transfer activity to l-O-alkyl-glycero-3-phosphorylcholine was estimated to be significantly lower than that to 1-acyl- and 1-O-alkenyl analogues in the same tissue. 3. The acyl transfer rates with different CoA thiol esters were highly specific to poly-unsaturated fatty acyl-CoA in contrast to acyl-CoA: 1-acyl-analogue acyltrans-ferase having activity to both saturated and unsaturated derivatives.