Metabolism of isoprenaline in the intestine

Abstract

A method for the study of drug metabolism in isolated canine gut loops is described. This technique has been used to study the conjugation of isoprenaline with ethereal sulphate. After intraluminal injection of tritiated isoprenaline only 3·6% of the radioactivity in venous effluent appeared as free isoprenaline, and 68·0% as a sulphate conjugate. After pretreatment with salicylamide the proportion of free isoprenaline rose to 73·7% and only 3·3% was accounted for as sulphate conjugate. The significance of these findings is discussed in relation to a possible source of drug interaction.