Novel Conformation of an RNA Structural Switch
Open Access
- 12 November 2012
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 51 (46), 9257-9259
- https://doi.org/10.1021/bi301372t
Abstract
The RNA duplex, (5'GACGAGUGUCA)(2), has two conformations in equilibrium. The nuclear magnetic resonance solution structure reveals that the major conformation of the loop, 5'GAGU/3'UGAG, is novel and contains two unusual Watson-Crick/Hoogsteen GG pairs with G residues in the syn conformation, two A residues stacked on each other in the center of the helix with inverted sugars, and two bulged-out U residues. The structure provides a benchmark for testing approaches for predicting local RNA structure and a sequence that allows the design of a unique arrangement of functional groups and/or a conformational switch into nucleic acids.Keywords
Funding Information
- National Science Council Taiwan
- National Institutes of Health
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