A rapid deprotection procedure for phosphotriester DNA synthesis
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (17), 6853-6859
- https://doi.org/10.1093/nar/12.17.6853
Abstract
An equimolar solution of aldoxime and tetramethylguanidine at 70 degrees C removes both the base and phosphate protection from oligonucleotides prepared by solid phase phosphate triester technology. The rate of cleavage from the succinyl linkage commonly used for solid phase synthesis is also increased. The method is simpler, faster and more easily automated than existing methods.Keywords
This publication has 3 references indexed in Scilit:
- Improvements to solid phase phosphotriester synthesis of deoxyoligonucleotidesNucleic Acids Research, 1982
- Some observations relating to the oximate ion promoted unblocking of oligonucleotide aryl estersNucleic Acids Research, 1981
- DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mappingNucleic Acids Research, 1974