The Effects of Metal Ions on Esterase Activities of Urokinase
- 31 December 1977
- journal article
- research article
- Published by Georg Thieme Verlag KG in Thrombosis and Haemostasis
- Vol. 39 (01), 046-052
- https://doi.org/10.1055/s-0038-1646653
Abstract
The esterase activity of highly purified human urokinase on Nα-acetylglycyl-L-lysine methyl ester is strongly inhibited by 1 × 10-5tol × 10“2MCu++, Hg++, Ni++, Co++, Fe+++, and Mn++ solutions, whereas Na+, K+, Ca++, and Mg++ are weakly effective. This inhibition is parallel with the inhibition of activation of plasminogen by urokinase. There is no simple linear relation between inhibition and ion concentration. Addition of ethylenediaminetetraacetate or electrodialysis fully reactivates the inhibited enzyme. These results are discussed in relation to similar effects of ions on trypsin.This publication has 10 references indexed in Scilit:
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