Properties of the ribosome-inactivating proteins gelonin, Momordica charantia inhibitor, and dianthins
- 1 December 1982
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 207 (3), 505-509
- https://doi.org/10.1042/bj2070505
Abstract
The amino acid and sugar compositions of 4 [phytoalexin] ribosome-inactivating proteins (gelonin, M. charantia inhibitor, dianthin 30 and dianthin 32) were determined. The proteins are all basic glycoproteins (pI > 8) containing mannose (more abudnant in gelonin), glucose, xylose, fuxose (absent from gelonin) and glucosamine. The ribosome-inactivating properties of the proteins examined are not modified by pretreatment with N-ethylmaleimide. Precipitating and inactivating antibodies can be raised against ribosome-inactivating proteins; a weak cross-reaction occurred only between dianthin 30 and dianthin 32.This publication has 14 references indexed in Scilit:
- Mannose 6-, fructose 1-, and fructose 6-phosphates inhibit human natural cell-mediated cytotoxicity.Proceedings of the National Academy of Sciences, 1981
- Cytotoxicity Acquired by Conjugation of an Anti‐Thy1.1 Monoclonal Antibody and the Ribosome‐Inactivating Protein, GeloninEuropean Journal of Biochemistry, 1981
- Purification and properties of a second antiviral protein from Phytolacca americana which inactivates eukaryotic ribosomesArchives of Biochemistry and Biophysics, 1980
- Inhibition of protein synthesis in vitro by proteins from the seeds of Momordica charantia (bitter pear melon)Biochemical Journal, 1980
- Purification and properties of a translation inhibitor from wheat germBiochemistry, 1979
- [34] Toxic and nontoxic lectins from Abrus precatoriusMethods in Enzymology, 1978
- The quantitation of glucosamine and galactosamine in glycoproteins after hydrolysis in p‐toluenesulphonic acidFEBS Letters, 1975
- A protein, immunologically similar to Tamm-Horsfall glycoprotein, produced by cultured baby hamster kidney cellsProceedings of the Royal Society of London. B. Biological Sciences, 1974
- The effect of an antiviral peptide on the ribosomal reactions of the peptide elongation enzymes, EF-I and EF-IIArchives of Biochemistry and Biophysics, 1973
- SYNTHESIS OF HEMOGLOBIN IN A CELL-FREE SYSTEM .1. PROPERTIES OF COMPLETE SYSTEM1962