AN INDIRECT HAEMAGGLUTINATION TEST FOR DEMONSTRATION OF GONOCOCCAL ANTIBODIES USING GONOCOCCAL PILI AS ANTIGEN

Abstract

Serum specimens (1223) were obtained from 649 consecutive patients attending a dermato-venereological out-patient clinic in Copenhagen with a request for venereal disease control. The sera were examined for gonococcal antibodies by a gonococcal complement fixation test (GCF) and an indirect hemagglutination test using gonococcal pili as antigen (IHA). The diagnosis of current gonococcal infection in 28% of the patients was based on positive culture for Neisseria gonorrhoeae from 1 or more of the following sites: urethra, rectum and fauces in all patients, and/or cervix in female patients. The specificity, sensitivity and predictive values of positive and negative test results were calculated for the various groups of patients on the assumption that all positive results, both in patients without gonorrhea but with a previous gonococcal infection and in patients without known current or previous infection, were false positives. The following values were found: sensitivity: IHA 45-100%, GCF 6-29%; specificity: IHA 65-89%, GCF 97-100%; predictive value of positive test result: IHA 43-74%, GCF 69-100%; predictive value of negative test result: IHA 78-100%, GCF 61-85%.