Expression in Escherichia coli of a New Multidrug Efflux Pump, MexXY, from Pseudomonas aeruginosa
- 1 February 1999
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 43 (2), 415-417
- https://doi.org/10.1128/aac.43.2.415
Abstract
Two new genes (mexXY) similar to mexAB,mexCD, and mexEF and mediating multidrug resistance were cloned from the chromosome of Pseudomonas aeruginosa. Elevated ethidium extrusion was observed withEscherichia coli cells harboring the plasmid carryingmexXY. This MexXY system confers higher resistance to fluoroquinolones than the MexAB and MexCD systems, and E. coli TolC or P. aeruginosa OprM is necessary for the function of the MexXY system.Keywords
This publication has 26 references indexed in Scilit:
- Characterization of MexE–MexF–OprN, a positively regulated multidrug efflux system of Pseudomonas aeruginosaMolecular Microbiology, 1997
- Overexpression of the mexC–mexD–oprJ efflux operon in nfxB‐type multidrug‐resistant strains of Pseudomonas aeruginosaMolecular Microbiology, 1996
- Genes acrA and acrB encode a stress‐induced efflux system of Escherichia coliMolecular Microbiology, 1995
- Efflux pumps and drug resistance in Gram-negative bacteriaTrends in Microbiology, 1994
- Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdineMolecular Microbiology, 1993
- EnvC, a new lipoprotein of the cytoplasmic membrane ofEscherichia coliFEMS Microbiology Letters, 1993
- Analysis of membrane and surface protein sequences with the hydrophobic moment plotJournal of Molecular Biology, 1984
- Studies on transformation of Escherichia coli with plasmidsJournal of Molecular Biology, 1983
- DNA sequence of the gene for the outer membrane lipoprotein of E. coli: an extremely AT-rich promoterCell, 1979
- Isolation of high molecular weight DNA from Hemophilus influenzaeJournal of Molecular Biology, 1965