The immunochemistry of Salmonella chemotype VI O-antigens. The structure of oligosaccharides from Salmonella group U (o 43) lipopolysaccharides

Abstract

A series of oligosaccharides was isolated from Salmonella milwaukee lipopolysaccharide by partial acid hydrolysis. Structural studies on these oligosaccharides indicated that the O-specific side chain of this lipopolysaccharide has a repeating pentasaccharide unit that is probably [alpha] -D-galactosyl- (1[forward arrow]3) -[beta] -D-galactosyl- (1 [forward arrow] 3) -N-acetylga-lactosaminyl-(1[forward arrow]3)-N-acetyl-D-glucosaminyl-(1[forward arrow]4)-L-fucose. Another oligosaccharide, which is not structurally related to the repeating pentasaccharide unit, has also been isolated and it is indistinguishable from an oligosaccharide obtained from Salmonella "rough" (R) lipopoly-saccharides. The isolation of this and similar core olisogaccharides from all chemotype VI lipopolysaccharides supports the view that Salmonella S-lipopolysaccharides have a common core that is probably identical with RII lipopolysaccharide.