COMPARISON OF HUMAN POLYCYSTIC AND MEDULLARY CYSTIC KIDNEY-DISEASE WITH DIPHENYLAMINE-INDUCED CYSTIC-DISEASE

Abstract

Because of difficulty in obtaining cystic human kidneys for functional and morphologic study, animal models are receiving increasing attention. Most prominent among them is the renal cystic disease that is induced in rats through feeding of the antioxidant, diphenylamine. The present study examined the morphology of adult polycystic and medullary cystic kidney disease in man using scanning electron microscopy and compared them with diphenylamine-induced cystic kidney disease in rats. Diphenylamine nephropathy was induced by feeding rats 1% diphenylamine for 12-18 mo. All types of cystic disease showed changes in the renal corpuscles, including dilatin of Bowman''s space, podocyte fusion and degeneration and basement membrane thickening. Cysts were noted along the entire nephron in polycystic and diphenylamine-induced cystic disease but only along the collecting ducts in medullary cystic disease. Cysts in polycystic disease were large and lined by flattened epithelium. Collecting duct cysts in diphenylamine cystic disease were lined by cells of irregular size and shape suggestive of cell hypertrophy and/or hyperplasia, whereas cysts of medullary cystic disease were lined by flattened epithelium except where the nephron entered or left the cyst. Cast material was generally found in the cysts of diphenylamine-induced and polycystic kidney disease but not medullary cystic disease. Atrophic glomeruli and tubules were found in all 3 diseases. Complete tubular obstruction was found in none; however, larger cysts frequently impinged on adjacent tubules and narrowed their lumina. In the terminal stages, cellular as well as gross morphologic differences exist between polycystic and medullary cystic kidney disease and diphenylamine-induced cystic disease more closely resembles human polycystic than medullary cystic kidney disease.